Improved approach to RNA and protein recognition for pathogen detection

Brian N. Zeiler; Burt V. Bronk; Abraham Grossman

doi:10.1117/12.394055

28 July 2000 Improved approach to RNA and protein recognition for pathogen detection

Brian N. Zeiler, Burt V. Bronk, Abraham Grossman

Proceedings Volume 4036, Chemical and Biological Sensing; (2000) https://doi.org/10.1117/12.394055
Event: AeroSense 2000, 2000, Orlando, FL, United States

Abstract

We are developing (U.S. Air Force, SBIR) a proprietary technology, based on the enzyme Q-beta replicase, to very rapidly detect and identify microorganisms (rRNA), virions (particularly RNA-based), and proteins of interest for pathogen detection. This enzyme is known to amplify a specific RNA signal one billion-fold in less than fifteen minutes at a constant temperature of 37 degree(s)C. RNA probes are made in `halves' that are joined to each other after their terminal ends are brought into proximity mediated by the binding to the target. Only such a full-length molecule can be amplified. We have demonstrated specific examples of recognition of RNA target sequences of interest in species of Bacillus and are investigating methods for overcoming the well-known promiscuity of the enzyme so that this recognition feature can be utilized with confidence, even in the presence of dirty backgrounds.

Citation Download Citation

Brian N. Zeiler, Burt V. Bronk, and Abraham Grossman "Improved approach to RNA and protein recognition for pathogen detection", Proc. SPIE 4036, Chemical and Biological Sensing, (28 July 2000); https://doi.org/10.1117/12.394055

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