Augmentation of tumor immunity with ENHANZYN adjuvant following verteporfin PDT: photodynamic vaccination (PDV)

P. Mark Curry; A. J. Stewart; L. Hardwicke; Claire Smits; John R. North

doi:10.1117/12.434705

9 July 2001 Augmentation of tumor immunity with ENHANZYN adjuvant following verteporfin PDT: photodynamic vaccination (PDV)

P. Mark Curry, A. J. Stewart, L. Hardwicke, Claire Smits, John R. North

Author Affiliations +

Proceedings Volume 4257, Laser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical; (2001) https://doi.org/10.1117/12.434705
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

The immune system is implicated in the mechanism of tumor destruction following photodynamic therapy (PDT). Several investigators have shown that immune stimulation can augment PDT. In this study, a single intratumoral injection of ENHANZYN^TM adjuvant was administered to tumor-bearing mice immediately following verteporfin PDT in a therapeutic modality referred to as Photodynamic Vaccination (PDV). After optimal PDT, little difference in the rate of tumor re-growth or time to tumor reappearance was seen upon addition of the adjuvant. This may be as expected as this treatment regimen results in effective long-term tumor cure in mice. The effect of adjuvant and sub-optimal PDT was less clear as both groups treated with either a high or low does of adjuvant showed tumor re-growth earlier than those animals treated with PDT alone. However, tumors of mice receiving sub-optimal PDT followed by high dose immune adjuvant did not show the rapid, uncontrolled growth seen in other groups and, in the majority of cases, tumor volume decreased steadily with time. This resulted in a superior period of survival despite the animals being tumor-bearing. Interestingly, the data obtained in this study clearly demonstrates the ability of PDT to protect against re- challenge with a second round of tumor implantation. This was seen in all groups and stresses the importance of the immune response in PDT tumor control. Addition of the high immune adjuvant does to sub-optimal PDT appeared to be the most effective treatment group in this respect, giving complete protection against tumor re-implantation.

Citation Download Citation

P. Mark Curry, A. J. Stewart, L. Hardwicke, Claire Smits, and John R. North "Augmentation of tumor immunity with ENHANZYN adjuvant following verteporfin PDT: photodynamic vaccination (PDV)", Proc. SPIE 4257, Laser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical, (9 July 2001); https://doi.org/10.1117/12.434705

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