Physicist's simple access to protein structures: the computer program WHAT IF

Brigitte Altenberg-Greulich; Stephan G. Zech; Dietmar Stehlik; Gert Vriend

doi:10.1117/12.432914

29 June 2001 Physicist's simple access to protein structures: the computer program WHAT IF

Brigitte Altenberg-Greulich, Stephan G. Zech, Dietmar Stehlik, Gert Vriend

Proceedings Volume 4430, ROMOPTO 2000: Sixth Conference on Optics; (2001) https://doi.org/10.1117/12.432914
Event: ROMOPTO 2000: Sixth Conference on Optics, 2000, Bucharest, Romania

Abstract

We describe the computer program WHAT IF and its application to two physical examples. For the DNA binding protein, OCT-1 (pou domain) the location of amino acids with a sidechain amino group is shown. Such knowledge is required when staining this molecule with a fluorescence dye, which binds chemically to the amino terminus as well as amino groups in sidechains. The program shows that most sidechain amino groups are protected when DNA is bound to OCT-1, allowing selective staining of the amino terminal NH2 group. A protein stained this way can be used in fluorescence spectroscopic studies on function aspects of OCT-1.

Citation Download Citation

Brigitte Altenberg-Greulich, Stephan G. Zech, Dietmar Stehlik, and Gert Vriend "Physicist's simple access to protein structures: the computer program WHAT IF", Proc. SPIE 4430, ROMOPTO 2000: Sixth Conference on Optics, (29 June 2001); https://doi.org/10.1117/12.432914

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