Advanced optical design for biochip scanning system

Guoliang Huang; Jing Cheng; Yuxiang Zhou; Jihong Feng; Chengxun Liu; Guofan Jin; Minxian Wu; Yingbai Yan; Rong Yang; Lin Li

doi:10.1117/12.449564

5 December 2001 Advanced optical design for biochip scanning system

Guoliang Huang, Jing Cheng, Yuxiang Zhou, Jihong Feng, Chengxun Liu, Guofan Jin, Minxian Wu, Yingbai Yan, Rong Yang, Lin Li

Author Affiliations +

Proceedings Volume 4441, Current Developments in Lens Design and Optical Engineering II; (2001) https://doi.org/10.1117/12.449564
Event: International Symposium on Optical Science and Technology, 2001, San Diego, CA, United States

Abstract

Laboratory-on-a-chip has been interested widely in recent years, where the sample preparation, bio-chemical reaction, separation, detection and analysis, are performed in a small biochip which is only a fingernall dimension. In order to obtain a high detection sensitivity 1 fluors/micrometers ² (one fluorescence molecular per square micrometer) in biochip scanning system, it is required that the scanning objective lens is a big numerical aperture (> 0.5), very small focal spot (< 5 micrometers ) and long back focal length (> 3 mm). In this paper, a combined lens is designed for the scanning objective lens, which is with big numerical aperture NA > 0.7, very small focal spot (< 2 micrometers ) and long back focal length (> 3 mm). The phase aberrations of combined lens, including the aspherical aberration and the chromatic aberration corresponding to wavelength 532 nm, 570 nm, 635 nm, 670 nm, are corrected very well. The encircled energy diagram of the lens is good to the diffraction limit. The focal spot diagram, the optical path difference diagram, the transverse ray fan plot and the modulation transfer function, are studied also. A novel confocal scanning system of biochip with the designed combined lens as the objective lens is developed, some experiment results in a multi-channel biochip are obtained.

Citation Download Citation

Guoliang Huang, Jing Cheng, Yuxiang Zhou, Jihong Feng, Chengxun Liu, Guofan Jin, Minxian Wu, Yingbai Yan, Rong Yang, and Lin Li "Advanced optical design for biochip scanning system", Proc. SPIE 4441, Current Developments in Lens Design and Optical Engineering II, (5 December 2001); https://doi.org/10.1117/12.449564

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available