5-HT spatial distribution imaging with multiphoton excitation of 5-HT correlative visible fluorescence in live cells

Zhihong Zhang; Shaoqun Zeng; Yafeng Liu; Wei Zhou; Tongsheng Chen; Qingming Luo

doi:10.1117/12.462565

12 April 2002 5-HT spatial distribution imaging with multiphoton excitation of 5-HT correlative visible fluorescence in live cells

Zhihong Zhang, Shaoqun Zeng, Yafeng Liu, Wei Zhou, Tongsheng Chen, Qingming Luo

Author Affiliations +

Proceedings Volume 4536, International Workshop on Photonics and Imaging in Biology and Medicine; (2002) https://doi.org/10.1117/12.462565
Event: International Workshop on Photonics and Imaging in Biology and Medicine, 2001, Wuhan, China

Abstract

The autofluorescence of 5-Hydroxytryptamine (5-HT) loaded rat mucosal mast cells (RBL-2H3 cells) is imaged with multiphoton excitation laser scanning microscope (MPELSM). 5-HT correlative visible fluorescence (F_co-vis) excited with 740-nm multiphoton excitation is observed in live cells for the first time, and the generating mechanism of 5-HT F_co-vis is studied. The spatial distribution of 5-HT in live cells is imaged at high spatial resolution in our experiment, which provides a new way to study the correlation between 5-HT spatial distribution and content, and the cellular functional state in live tissue or cells.

Citation Download Citation

Zhihong Zhang, Shaoqun Zeng, Yafeng Liu, Wei Zhou, Tongsheng Chen, and Qingming Luo "5-HT spatial distribution imaging with multiphoton excitation of 5-HT correlative visible fluorescence in live cells", Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); https://doi.org/10.1117/12.462565

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