Fluorescence micro-imaging of living cells and biomolecules with ultrahigh NA objectives

Susumu Terakawa; Takashi Tsuboi; Takashi Sakurai; Andreas Jeromin; Yoshihiko Wakazono; Seiji Yamamoto; Katsuyuki Abe

doi:10.1117/12.446647

25 October 2001 Fluorescence micro-imaging of living cells and biomolecules with ultrahigh NA objectives

Susumu Terakawa, Takashi Tsuboi, Takashi Sakurai, Andreas Jeromin, Yoshihiko Wakazono, Seiji Yamamoto, Katsuyuki Abe

Proceedings Volume 4597, Biophotonics Instrumentation and Analysis; (2001) https://doi.org/10.1117/12.446647
Event: International Symposium on Photonics and Applications, 2001, Singapore, Singapore

Abstract

Optical imaging with a high detecting power is very instrumental to dynamic observations of bio-molecular objects in water. To pioneer this field of imaging researches further, we used novel objective lenses of 1.65 and 1.45 in NA comparatively with 1.35 NA lens. The lenses of ultra high NA have a high resolving power and very useful properties for observation of high contrast images of fluorescently labeled molecules and subcellular organelles including DNA and membrane fusion proteins.

Citation Download Citation

Susumu Terakawa, Takashi Tsuboi, Takashi Sakurai, Andreas Jeromin, Yoshihiko Wakazono, Seiji Yamamoto, and Katsuyuki Abe "Fluorescence micro-imaging of living cells and biomolecules with ultrahigh NA objectives", Proc. SPIE 4597, Biophotonics Instrumentation and Analysis, (25 October 2001); https://doi.org/10.1117/12.446647

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