Fluorescence photobleaching and recovery of human breast tissues and tissue phantoms

Sharad Gupta; Bhawna Bhawna; Asima Pradhan; S. Swain; Asha Agarwal

doi:10.1117/12.465262

1 May 2002 Fluorescence photobleaching and recovery of human breast tissues and tissue phantoms

Sharad Gupta, Bhawna Bhawna, Asima Pradhan, S. Swain, Asha Agarwal

Proceedings Volume 4613, Optical Biopsy IV; (2002) https://doi.org/10.1117/12.465262
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Changes in the fluorescence intensity at 530 nm and 630 nm are measured as a function of incident intensity and exposure time with 488 nm excitation of Argon ion laser. Photobleaching curves of normal and diseased tissues display double exponential behaviour with different decay rates. Photobleaching rates of fluorophores in solutions mostly display single exponential behaviour while addition of absorber and scatterers results in double exponential profiles. The decay profile of FAD + Protoporphyrin indicates the contribution of both fluorophores in tissue photobleaching at 530 nm. Photobleaching profiles of normal tissues with blood show a fast decay in comparison to the normal and diseased tissues without blood. The fast decay rates of tumors indicate absorption of flavin by porphyrin. Fluorescence photobleaching recovery (FPR) profiles of tissue displays single exponential behaviour but the rates of growth of normal and diseased tissues are different.

Citation Download Citation

Sharad Gupta, Bhawna Bhawna, Asima Pradhan, S. Swain, and Asha Agarwal "Fluorescence photobleaching and recovery of human breast tissues and tissue phantoms", Proc. SPIE 4613, Optical Biopsy IV, (1 May 2002); https://doi.org/10.1117/12.465262

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