Use of steady-state fluorescence anisotropy with pebble nanosensors for chemical analysis

Thomas Horvath; Eric E. Monson; James Sumner; Hao Xu; Raoul Kopelman

doi:10.1117/12.472115

21 June 2002 Use of steady-state fluorescence anisotropy with pebble nanosensors for chemical analysis

Thomas Horvath, Eric E. Monson, James Sumner, Hao Xu, Raoul Kopelman

Proceedings Volume 4626, Biomedical Nanotechnology Architectures and Applications; (2002) https://doi.org/10.1117/12.472115
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

In this study we show that steady-state fluorescence anisotorpy within PEBBLEs can be used for the optochemical sensing of analytes such as Zn²⁺, O₂, and Ca²⁺. Steady-state fluorescence anisotropy is a non- time resolved method that measures a combination of rotational and fluorescence lifetimes. This eliminates the need for reference dyes and ratiometic techniques to obtain quantitative results, even when using intensity-based sensor dyes. An advantage to working with PEBBLE nanosensors is that the encapsulated dye is localized in a constant rotational environment. This is in contrast to the use of free dyes, which can be affected by interferents such as protein binding.

Citation Download Citation

Thomas Horvath, Eric E. Monson, James Sumner, Hao Xu, and Raoul Kopelman "Use of steady-state fluorescence anisotropy with pebble nanosensors for chemical analysis", Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); https://doi.org/10.1117/12.472115

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