Results of UV laser application on biological material

P. Alifano; Vincenzo Nassisi; Pier Paolo Pompa; A. Candido

doi:10.1117/12.478656

9 August 2002 Results of UV laser application on biological material

P. Alifano, Vincenzo Nassisi, Pier Paolo Pompa, A. Candido

Proceedings Volume 4762, ALT'01 International Conference on Advanced Laser Technologies; (2002) https://doi.org/10.1117/12.478656
Event: International Conference on: Advanced Laser Technologies (ALT'01), 2001, Constanta, Romania

Abstract

In this paper we report on the biological effects of XeCL laser irradiation on Staphylococcus epidermidis and Escherichia coli. UV interaction with cellular systems is responsible for photochemical, photothermal or photodecomposition processes. When short-wavelength UV radiation strikes biological material, the DNA is damaged causing cell killing, mutagenesis or carcinogenesis. We report on different effects of XeCl laser irradiation on two microbial systems; collection strain of Staphylococcus epidermidis (in suspension) and collection strains of Eschericha coli proficient or deficient in DNA recombination/repair pathways (irradiated on solid surfaces). In S epidermidis the 308 nm radiation can significantly enhanced the proliferation rates. In wild type E. coli cells the radiation did not stimulate the growth rates. Surprisingly, the 308 nm radiation elicited a very strong lethal effect on DNA recombination/repair-defective strains (harbouring the recA56 null mutation), even more pronounced than irradiation with a UV 254 nm germicidal lamp. The unknown mechanism responsible for this biological response is currently under investigation.

Citation Download Citation

P. Alifano, Vincenzo Nassisi, Pier Paolo Pompa, and A. Candido "Results of UV laser application on biological material", Proc. SPIE 4762, ALT'01 International Conference on Advanced Laser Technologies, (9 August 2002); https://doi.org/10.1117/12.478656

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