Time-delayed fluorescence imaging of a porphycene derivative

Sarah L. Gundy; Wilhelm J.M. van der Putten; Andrew Shearer; Daniel J. Buckton; Alan G. Ryder; Michael Ball

doi:10.1117/12.473596

13 June 2003 Time-delayed fluorescence imaging of a porphycene derivative

Sarah L. Gundy, Wilhelm J.M. van der Putten, Andrew Shearer, Daniel J. Buckton, Alan G. Ryder, Michael Ball

Proceedings Volume 4952, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XII; (2003) https://doi.org/10.1117/12.473596
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Porphycenes are currently under investigation for use in Photodynamic therapy, which is a promising treatment for cancer. These materials, which display preferential uptake in cancerous cells, also exhibit high fluorescence yields, and can be used for tumour detection. Problems with steady-state fluorescence techniques such as background autofluorescence can be eliminated by the use of time-resolved techniques. Improved contrast can be obtained with time-resolved techniques because of the differing fluorescence lifetimes between autofluorescence and longer-living exogenous photosensitisers. An imaging system was constructed using a fast (200 ps) gated CCD camera and a pulsed 635 nm laser diode. A tissue phantom composed of polymethyl methacrylate (PMMA) with thirty-six wells of varying diameter and depth (10 mm to 1 mm) was assembled to test the system. The system was used to record images of a porphycene derivative within the wells at differing concentrations in an organic solvent. A tissue imitator was placed on top of the PMMA block at varying thickness. 10-4 M zinc phthalocyanine tetrasulfonate was also placed on top of the block to mimic autofluorescence. The results indicate that the time-gated imaging system can prevent background excitation scatter and fluorescence from a shorter-lived fluorophore from distorting the fluorescence signal from a longer-lived photosensitiser.

Citation Download Citation

Sarah L. Gundy, Wilhelm J.M. van der Putten, Andrew Shearer, Daniel J. Buckton, Alan G. Ryder, and Michael Ball "Time-delayed fluorescence imaging of a porphycene derivative", Proc. SPIE 4952, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XII, (13 June 2003); https://doi.org/10.1117/12.473596

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