NIR imaging the delivery of cathepsin B probe to breast tumors

Lanlan Zhou; Dana M. Blessington; Zhihong Zhang; Aristid E. Lindenmayer; Ching Hsuan Tung; Ralph Weissleder; Britton Chance

doi:10.1117/12.478188

29 July 2003 NIR imaging the delivery of cathespin B probe to breast tumors

Lanlan Zhou, Dana M. Blessington, Zhihong Zhang, Aristid E. Lindenmayer, Ching Hsuan Tung, Ralph Weissleder, Britton Chance

Author Affiliations +

Proceedings Volume 4955, Optical Tomography and Spectroscopy of Tissue V; (2003) https://doi.org/10.1117/12.478188
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Proteases are involved in the invasion and metastasis of tumor cells. Cathepsin B overexpression has been shown in some neoplastic tissues. This study assesses the expression of Cathepsin B in the human fibrosarcoma (HT1080) in the mouse model by near-infrared (NIR) imaging. The nude mice were intravenously injected "a stealth probe" - an activable Cathepsin B sensing near-infrared fluorescence (NIRF) probe (24 hours before sacrifice) and the dye Cy5.5 (30 seconds before sacrifice). The animals were freeze-trapped and NIR images were obtained by the low temperature NIR scanner at the following excitation-emission wavelength pairs: 366, 450nm (NADH), 436, 520nm (FAD), and 670, 695nm (Cathepsin B probe). After imaging, the samples were submitted for histopathological evaluation. The tumor redox ratio NADH/(NADH+FAD) increased significantly because of the hypoxic state of tumor tissue with respect to normal tissue. The Cathepsin B probe was uniformly distributed throughout the tumor. This study indicated the efficient usage of the Cathepsin B probe in the molecular imaging for the detection of the early stage tumors.

Citation Download Citation

Lanlan Zhou, Dana M. Blessington, Zhihong Zhang, Aristid E. Lindenmayer, Ching Hsuan Tung, Ralph Weissleder, and Britton Chance "NIR imaging the delivery of cathespin B probe to breast tumors", Proc. SPIE 4955, Optical Tomography and Spectroscopy of Tissue V, (29 July 2003); https://doi.org/10.1117/12.478188

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