Compact time-resolved laser-induced fluorescence spectroscopic system for clinical investigations of diseased tissues

Qiyin Fang; Thanassis Papaioannou; Javier Jo; Russel Vaitha; Kumar Shastry; Laura Marcu

doi:10.1117/12.476133

22 July 2003 Compact time-resolved laser-induced fluorescence spectroscopic system for clinical investigations of diseased tissues

Qiyin Fang, Thanassis Papaioannou, Javier Jo, Russel Vaitha, Kumar Shastry, Laura Marcu

Author Affiliations +

Proceedings Volume 4958, Advanced Biomedical and Clinical Diagnostic Systems; (2003) https://doi.org/10.1117/12.476133
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

In this communication, we report the design, development, calibration, and characterization of a fluorescence spectroscopy instrument capable of measuring time-resolved fluorescence spectra from 350nm to 800nm with subnanosecond resolution. The compact system can be used in a variety of clinical settings including endoscopic procedures. The instrument can accommodate various types of laser sources and fiber optic probes for different types of applications. The typical acquisition time is about 0.8 s per wavelength or 40 s for a 200 nm time-resolved fluorescence spectrum including online fluorescence lifetime analysis. The system has a variable spectral resolution from 0.1 to 10 nm and temporal resolution of 300 ps. Fluorescence spectra can be acquired with a sensitivity of 10^-9M at signal-to-noise ratio of 46. The fluorescence lifetimes of Rhodamin B and 9- cyanoanthracene were determined to be 2.92∓0.12 ns and 12.28∓0.12 ns indicating good agreement with literature values.

Citation Download Citation

Qiyin Fang, Thanassis Papaioannou, Javier Jo, Russel Vaitha, Kumar Shastry, and Laura Marcu "Compact time-resolved laser-induced fluorescence spectroscopic system for clinical investigations of diseased tissues", Proc. SPIE 4958, Advanced Biomedical and Clinical Diagnostic Systems, (22 July 2003); https://doi.org/10.1117/12.476133

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KEYWORDS

Luminescence

Time resolved spectroscopy

Data acquisition

Laser induced fluorescence

Spectrographs

Fluorescence spectroscopy

Fiber optics

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