In situ identification of bacteria size by light scattering

Alvin Katz; Alexandra Alimova; Min Xu; Elizabeth Rudolph; Howard E. Savage; Mahendra Shah; Steven A. McCormick M.D.; Richard B. Rosen M.D.; Robert R. Alfano

doi:10.1117/12.479188

23 July 2003 In situ identification of bacteria size by light scattering

Alvin Katz, Alexandra Alimova, Min Xu, Elizabeth Rudolph, Howard E. Savage, Mahendra Shah, Steven A. McCormick M.D., Richard B. Rosen M.D., Robert R. Alfano

Author Affiliations +

Proceedings Volume 4965, Optical Diagnostics and Sensing in Biomedicine III; (2003) https://doi.org/10.1117/12.479188
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Optical transmission and light scattering measurements were performed on three species of bacteria with different sizes and shapes (Pseudomonas aeruginosa. Staphylococcus aureus and Bacillus subtilis). The average bacteria size was determined from transmission measurements by using the Gaussian Ray Approximation of Anomalous Diffraction Theory. A rescaled spectra combining multiple angular data was analyzed in the framework of the Rayleigh-Gans theory of light scattering in order to determine particle shape and size distribution. Particle size and shape as determined by both methods are in good agreement with size and shape measured by scanning electron microscopy. These results demonstrate that light scattering may be able to detect and identify microbial contamination in the environment.

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Alvin Katz, Alexandra Alimova, Min Xu, Elizabeth Rudolph, Howard E. Savage, Mahendra Shah, Steven A. McCormick M.D., Richard B. Rosen M.D., and Robert R. Alfano "In situ identification of bacteria size by light scattering", Proc. SPIE 4965, Optical Diagnostics and Sensing in Biomedicine III, (23 July 2003); https://doi.org/10.1117/12.479188

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