New two-photon fluorescent probe for multiphoton microscopy in biological media

Raz Gvishi; Garry Berkovic; Zvi Kotler; P. Krief; J. Y. Becker; M. Sigalov; Lev Shapiro; Vladimir Khodorkovsky

doi:10.1117/12.498451

8 July 2003 New two-photon fluorescent probe for multiphoton microscopy in biological media

Raz Gvishi, Garry Berkovic, Zvi Kotler, P. Krief, J. Y. Becker, M. Sigalov, Lev Shapiro, Vladimir Khodorkovsky

Proceedings Volume 5036, Photonics, Devices, and Systems II; (2003) https://doi.org/10.1117/12.498451
Event: Photonics, Devices, and Systems II, 2002, Prague, Czech Republic

Abstract

An important ingredient in improving Multi Photon Laser Scanning Microscopy, MPLSM, is the development of efficient two-photon fluorescent (TPF) probes. We previously reported on a new class of TPF probes, specifically designed in order to maximize their efficiency in potential MPLSM applications. The fluorophores are based on a tetraketo derivative (TK) with a symmetric structure Donor-Acceptor-Donor (D-A-D). Those fluorophores have the following properties: a) Very large two-photon absorption coefficients (δ ~ 1000GM); b) Two-photon excitation (TPE) peak wavelength strongly shifted to the red (λ ~ 1µm); c) High fluorescence quantum efficiency; d) Large Stokes shifts of the fluorescence bands. We extended our work to a new fluorophore from this class that is more suitable for biological settings. This new fluorophore has a structure of crown-TK-crown that incorporates the ability to trap metal ions such as calcium. The TPE wavelength dependence of the TK-crown derivative is very similar to its analogous linear derivative with enhancement in the value of the cross-section, due to the stronger donor moieties. The TPE cross-section for the TK-crown derivative was about δ = 950 GM at λ_max = 980 nm.

Citation Download Citation

Raz Gvishi, Garry Berkovic, Zvi Kotler, P. Krief, J. Y. Becker, M. Sigalov, Lev Shapiro, and Vladimir Khodorkovsky "New two-photon fluorescent probe for multiphoton microscopy in biological media", Proc. SPIE 5036, Photonics, Devices, and Systems II, (8 July 2003); https://doi.org/10.1117/12.498451

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