Optical tomography of pigmented human skin biopsies

Iris Riemann; Peter Fischer; Martin Kaatz; Tobias W. Fischer; Peter Elsner; Enrico Dimitrov; Annette Reif; Karsten Konig

doi:10.1117/12.528278

13 July 2004 Optical tomography of pigmented human skin biopsies

Iris Riemann, Peter Fischer, Martin Kaatz, Tobias W. Fischer, Peter Elsner, Enrico Dimitrov, Annette Reif, Karsten Konig

Author Affiliations +

Proceedings Volume 5312, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV; (2004) https://doi.org/10.1117/12.528278
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

The novel femtosecond NIR (near infrared) laser based high resolution imaging system DermaInspect was used for non-invasive diagnostics of pigmented skin. The system provides fluorescence and SHG images of high spatial submicron resolution (3D) and 250 ps temporal resolution (4D) based on time resolved single photon counting (TCSPC). Pigmented tissue biopsies from patients with nevi and melanoma have been investigated using the tunable 80 MHz femtosecond laser MaiTai with laser wavelengths in the range of 750 - 850 nm. The autofluorescence patterns of different intratissue cell types and structures were determined. The non-linear induced autofluorescence originates from naturally endogenous fluorophores and protein structures like NAD(P)H, flavins, elastin, collagen, phorphyrins and melanin. In addition to autofluorescence, SHG (second harmonic generation) was used to detect dermal collagen structures. Interestingly, pigmented cells showed intense luminescence signals. Further characterization of tissue components was performed via 4D measurements of the fluorescence lifetime (x, y, z, τ). The novel multiphoton technique offers the possibility of a painless high resolution non invasive diagnostic method (optical biopsy), in particular for the early detection of skin cancer.

Citation Download Citation

Iris Riemann, Peter Fischer, Martin Kaatz, Tobias W. Fischer, Peter Elsner, Enrico Dimitrov, Annette Reif, and Karsten Konig "Optical tomography of pigmented human skin biopsies", Proc. SPIE 5312, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV, (13 July 2004); https://doi.org/10.1117/12.528278

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