Protein-based molecular contrast optical coherence tomography

Changhuei Yang; Michael A. Choma; Laura E. Lamb; John D. Simon; Joseph A. Izatt

doi:10.1117/12.531353

1 July 2004 Protein-based molecular contrast optical coherence tomography

Changhuei Yang, Michael A. Choma, Laura E. Lamb, John D. Simon, Joseph A. Izatt

Proceedings Volume 5316, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VIII; (2004) https://doi.org/10.1117/12.531353
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

We describe a novel technique for contrast enhancement in optical coherence tomography (OCT) which uses optically switchable protein based chromophores. Photosensitive proteins, such as bacteriorhodopsin and phytochrome, are promising OCT molecular contrast agents by reason of their remarkably low transition activation intensities compatible with in vivo imaging, and their potential for use as genetically expressible markers for molecular imaging. This study details the use of a novel optical switch suppression scheme which uses the absorption change between the two state groups of phytochrome to extract concentration and distribution information of the contrast agent within a target sample.

Citation Download Citation

Changhuei Yang, Michael A. Choma, Laura E. Lamb, John D. Simon, and Joseph A. Izatt "Protein-based molecular contrast optical coherence tomography", Proc. SPIE 5316, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VIII, (1 July 2004); https://doi.org/10.1117/12.531353

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