Photopatterning of DNA oligonucleotides on silicon surfaces with micron-scale dimensions

Huabing B. Yin; Tom Brown; Robert Greef; Sakellaris Mailis; Rob Eason; James S. Wilkinson; Tracy Melvin

doi:10.1117/12.545736

8 September 2004 Photopatterning of DNA oligonucleotides on silicon surfaces with micron-scale dimensions

Huabing B. Yin, Tom Brown, Robert Greef, Sakellaris Mailis, Rob Eason, James S. Wilkinson, Tracy Melvin

Proceedings Volume 5461, Biophotonics New Frontier: From Genome to Proteome; (2004) https://doi.org/10.1117/12.545736
Event: Photonics Europe, 2004, Strasbourg, France

Abstract

A method is presented for the covalent attachment of oligonucleotides to silicon (100) surfaces patterned with micron-scale features. UV light exposure of hydrogen-terminated silicon (100) coated with alkenes functionalized with N-hydroxysuccinimide ester groups results in Si-C bonded monolayers. The N-hydroxysuccinimide ester surfaces act as a template for the subsequent covalent attachment of DNA oligonucleotides. In order to create patterns of surface attached DNA oligonucleotides with high density, the surface attachment chemistry has been investigated and optimised. Micron-scale patterning of surfaces was achieved by exposure with UV laser light via a mask. DNA oligonucleotide patterns, with feature sizes of several microns, were reliably produced over large areas. The patterned surfaces were characterised with scanning electron microscopy, epifluorescence microscopy and ellipsometry. Hybridisation with fluorescent label- and gold nanoparticle-conjugates of the complementary oligonucleotide is achieved. The methods offer reliable approaches for the creation of micron-scale motifs of DNA on surfaces.

Citation Download Citation

Huabing B. Yin, Tom Brown, Robert Greef, Sakellaris Mailis, Rob Eason, James S. Wilkinson, and Tracy Melvin "Photopatterning of DNA oligonucleotides on silicon surfaces with micron-scale dimensions", Proc. SPIE 5461, Biophotonics New Frontier: From Genome to Proteome, (8 September 2004); https://doi.org/10.1117/12.545736

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available