Two-photon excitation of chlorin-e6-C15 monomethyl ester for photodynamic therapy

Ping Chen D.D.S.; P. D. Zhao D.D.S.; P. Guo D.D.S.; Lie Lin D.D.S.; J. Wei Liu; Q. Yu

doi:10.1117/12.575941

18 January 2005 Two-photon excitation of chlorin-e₆-C₁₅ monomethyl ester for photodynamic therapy

Ping Chen D.D.S., P. D. Zhao D.D.S., P. Guo D.D.S., Lie Lin D.D.S., J. Wei Liu, Q. Yu

Proceedings Volume 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II; (2005) https://doi.org/10.1117/12.575941
Event: Photonics Asia, 2004, Beijing, China

Abstract

Two-photon-induced fluorescence spectrum and lifetime of Chlorin-e₆-C₁₅ Monomethyl Ester in tetrahydrofura (THF) are experimentally examined with femtosecond laser pulses at 800 nm from a Ti:sapphire laser. The two-photon excited fluorescence spectra of the molecule are basically similar to those obtained by one-photon excitation. The lifetimes of two-photon and one-photon excitation fluorescence of this molecule in the solution are of the order of 5.2 ns and 4.8 ns respectively. Our experimental results indicate that the two-photon-induced photodynamic processes of Chlorin-e₆-C₁₅ Monomethyl Ester are similar to one-photon-induced photodynamic processes. The two-photon absorption cross section of the molecule is measured at 800 nm as about σ₂^' ≈ 29.1 x 10^-50 cm⁴ • s/photon. As an example for two-photon photodynamic therapy, we also further examine the cell-damaging effects of the Ester. Our preliminary results of cell viability test indicate that Chlorin-e₆-C₁₅ Monomethyl Ester can effectively damage the liver cancer cells BEL-7402 under two-photon irradiation. Our results suggest Chlorin-e₆-C₁₅ Monomethyl Ester may become a potential two-photon phototherapeutic agent.

Citation Download Citation

Ping Chen D.D.S., P. D. Zhao D.D.S., P. Guo D.D.S., Lie Lin D.D.S., J. Wei Liu, and Q. Yu "Two-photon excitation of chlorin-e₆-C₁₅ monomethyl ester for photodynamic therapy", Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); https://doi.org/10.1117/12.575941

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