Position-sensitive scanning fluorescence correlation spectroscopy

Joseph P. Skinner; Yan Chen; Joachim D. Mueller

doi:10.1117/12.600823

30 March 2005 Position-sensitive scanning fluorescence correlation spectroscopy

Joseph P. Skinner, Yan Chen, Joachim D. Mueller

Proceedings Volume 5700, Multiphoton Microscopy in the Biomedical Sciences V; (2005) https://doi.org/10.1117/12.600823
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Most fluorescence fluctuation experiments use a stationary laser beam to illuminate a small sample volume and analyze the temporal behavior of the fluorescence fluctuations within the stationary observation volume. Scanning of the laser beam in a circular pattern collects the fluorescence signal from a moving observation volume. The fluctuations contain now information about temporal and spatial properties of the sample. Synchronization between beam scanning and data collection allows us to evaluate the fluctuations for every position along the scanned trajectory. We present the theory of position-sensitive scanning fluorescence fluctuation spectroscopy and experimentally verify the theory. This technique is useful for detecting and characterizing directed transport processes in the presence of diffusion.

Citation Download Citation

Joseph P. Skinner, Yan Chen, and Joachim D. Mueller "Position-sensitive scanning fluorescence correlation spectroscopy", Proc. SPIE 5700, Multiphoton Microscopy in the Biomedical Sciences V, (30 March 2005); https://doi.org/10.1117/12.600823

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