Data analysis considerations in probing single quantum dot fluorescence intermittency

Jeffrey R. Krogmeier; Jeeseong Hwang

doi:10.1117/12.590707

28 April 2005 Data analysis considerations in probing single quantum dot fluorescence intermittency

Proceedings Volume 5705, Nanobiophotonics and Biomedical Applications II; (2005) https://doi.org/10.1117/12.590707
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

The fluorescence intermittency of single, bare, CdSe/ZnS quantum dots was probed using single molecule confocal microscopy and found to demonstrate power law kinetics. Various threshold values and line fitting parameters are employed in the data analysis and their effects on the extracted power law exponents, m_off and m_on, are presented. The threshold is found to be critical in determining m_off while having no significant effect on m_on. The mean plus 2σ threshold, calculated from the background noise in the measurement, results in a more negative m_off slope in comparison to the mean plus 3σ or mean plus 4σ thresholds. This is likely due to the mean plus 2σ threshold lying within the background noise outliers which mimic short on events. In contrast, the mean plus 4σ threshold is above 99.99% of the background noise while adequately below the fluorescence signal. Additionally, it is found that fitting only the ten most probable data points rather than all the data points in the log-log probability density graphs results in no significant change in m_off and mon.

Citation Download Citation

Jeffrey R. Krogmeier and Jeeseong Hwang "Data analysis considerations in probing single quantum dot fluorescence intermittency", Proc. SPIE 5705, Nanobiophotonics and Biomedical Applications II, (28 April 2005); https://doi.org/10.1117/12.590707

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