Coherent control of mutiphoton excitation process for biological fluorescence imaging

Katsumi Midorikawa; Jianfan Chen; Hiroyuki Kawano; Yasuo Nabekawa; Akira Suda; Hideaki Mizuno; Atsushi Miyawaki

doi:10.1117/12.600034

21 March 2005 Coherent control of mutiphoton excitation process for biological fluorescence imaging

Katsumi Midorikawa, Jianfan Chen, Hiroyuki Kawano, Yasuo Nabekawa, Akira Suda, Hideaki Mizuno, Atsushi Miyawaki

Proceedings Volume 5714, Commercial and Biomedical Applications of Ultrafast Lasers V; (2005) https://doi.org/10.1117/12.600034
Event: Lasers and Applications in Science and Engineering, 2005, San Jose, California, United States

Abstract

The two-photon excitation fluorescence (TPEF) process of an enhanced green fluorescent protein (EGFP) for fluorescence signals was adaptively controlled by the phase-modulation of femtosecond pulses. After the iteration of pulse shaping, a twofold increase in the ratio of the fluorescence signal to the laser peak power was achieved. Compared with conventional pulses optimized for peak power, phase-optimized laser pulses reduced the bleaching rate of EGFP by a factor of 4 while maintaining the same intensity of the fluorescence signal. The ratio of two-photon (2P) fluorescence from EGFP and three-photon (3P) fluorescence from the essential amino acid L-Tryptophan was also enhanced by using the adaptive pulse shaping technique. To achieve a trade-off between the 2P/3P fluorescence ratio and the 2P fluorescence intensity, we then engineered the cost function in the self-learning algorithm. These methods have much potential for immediate application to various important biological and medical studies.

Citation Download Citation

Katsumi Midorikawa, Jianfan Chen, Hiroyuki Kawano, Yasuo Nabekawa, Akira Suda, Hideaki Mizuno, and Atsushi Miyawaki "Coherent control of mutiphoton excitation process for biological fluorescence imaging", Proc. SPIE 5714, Commercial and Biomedical Applications of Ultrafast Lasers V, (21 March 2005); https://doi.org/10.1117/12.600034

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available