PCR detection using nanofabricated SERS microchips

N. Elejalde; H. M. H. Chong; O. Inya-Agha; J. M. Cooper

doi:10.1117/12.599857

16 May 2005 PCR detection using nanofabricated SERS microchips

N. Elejalde, H. M. H. Chong, O. Inya-Agha, J. M. Cooper

Author Affiliations +

Proceedings Volume 5763, Smart Structures and Materials 2005: Smart Electronics, MEMS, BioMEMS, and Nanotechnology; (2005) https://doi.org/10.1117/12.599857
Event: SPIE Smart Structures and Materials + Nondestructive Evaluation and Health Monitoring, 2005, San Diego, California, United States

Abstract

This paper describes a novel method for the fabrication of nanodot arrays with 30nm period which will be used as a platform for the highly sensitive and specific Surface enhanced Raman spectroscopy (SERS) detection of the polymerase chain reaction (PCR). The usual detection methods for PCR involve time consuming methods of DNA labelling, using labels that are capable of altering original DNA properties. We present a detection method which has the advantages of being label free, requiring small analyte volumes and achieving high sensitivity due to SERS enhancement. The required reproducible SERS environment is achieved by the nanofabrication of gold pillars on glass with the use of an electron-beam writer.

Citation Download Citation

N. Elejalde, H. M. H. Chong, O. Inya-Agha, and J. M. Cooper "PCR detection using nanofabricated SERS microchips", Proc. SPIE 5763, Smart Structures and Materials 2005: Smart Electronics, MEMS, BioMEMS, and Nanotechnology, (16 May 2005); https://doi.org/10.1117/12.599857

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE