Comparison of the photodynamic effect in human and animal tumor cell lines

Elena Stoykova; Radostina Alexandrova; Kristina Nedkova; Elena Ivanova; Ognian Sabotinov; Kaloian Zdravkov; Georgi Minchev

doi:10.1117/12.618515

22 April 2005 Comparison of the photodynamic effect in human and animal tumor cell lines

Elena Stoykova, Radostina Alexandrova, Kristina Nedkova, Elena Ivanova, Ognian Sabotinov, Kaloian Zdravkov, Georgi Minchev

Author Affiliations +

Proceedings Volume 5830, 13th International School on Quantum Electronics: Laser Physics and Applications; (2005) https://doi.org/10.1117/12.618515
Event: 13th International School on Quantum Electronics: Laser Physics and Applications, 2004, Bourgas, Bulgaria

Abstract

The aim of the present work is to compare the photodynamic effect in vitro for permanent cell lines established from some of the most common and invasive human cancers (breast cancer and brain glioblastoma) as well as for animal cell lines obtained from virus-induced transplantable tumors. The cytotoxicity assessment was performed for human breast adenocarcinoma MCF-7, human glioblastoma 8-MG-BA, and two virus-induced animal tumor cell lines: a cell line LSCC-SF-Mc29, obtained from a transplantable chicken hepatoma induced by the myelocytomatosis virus Mc20, and a line LSR-SF- SR, obtained from a transplantable sarcoma in rat induced by Rous sarcoma virus strain Schmidt-Ruppin. We used in the experiments a PS produced by NIOPIK (Russia) [www.tech-db.ru/istc/db/inst.nsf/wu] with peak absorption around 670 nm. The photodynamic effect was assessed by a neutral red uptake cytotoxicity test. To activate the photosensitizer we used a semiconductor laser that emitted at 672 nm at irradiance of 120 mW/cm²; the latter value had been chosen after comparison of the photodynamic effect at 12, 60 and 120 mW/cm².

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Elena Stoykova, Radostina Alexandrova, Kristina Nedkova, Elena Ivanova, Ognian Sabotinov, Kaloian Zdravkov, and Georgi Minchev "Comparison of the photodynamic effect in human and animal tumor cell lines", Proc. SPIE 5830, 13th International School on Quantum Electronics: Laser Physics and Applications, (22 April 2005); https://doi.org/10.1117/12.618515

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