Sub-resolution axial localization of nanoparticles in fluorescence microscopy

Francois Aguet; Dimitri Van De Ville; Michael Unser

doi:10.1117/12.643899

7 October 2005 Sub-resolution axial localization of nanoparticles in fluorescence microscopy

Francois Aguet, Dimitri Van De Ville, Michael Unser

Proceedings Volume 5860, Confocal, Multiphoton, and Nonlinear Microscopic Imaging II; 58600P (2005) https://doi.org/10.1117/12.643899
Event: European Conferences on Biomedical Optics 2005, 2005, Munich, Germany

Abstract

We propose a method for sub-resolution axial localization of particles in fluorescence microscopy, based on maximum-likelihood estimation. Given acquisitions of a defocused fluorescent particle, we can estimate its axial position with nanometer range precision.

Citation Download Citation

Francois Aguet, Dimitri Van De Ville, and Michael Unser "Sub-resolution axial localization of nanoparticles in fluorescence microscopy", Proc. SPIE 5860, Confocal, Multiphoton, and Nonlinear Microscopic Imaging II, 58600P (7 October 2005); https://doi.org/10.1117/12.643899

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