Molecular binding and enhanced photoluminescence of bromocresol purple in marine derived DNA

Darnell E. Diggs; Josh Hagen; Zhou Yu; Emily Heckman; F. Kenneth Hopkins; James G. Grote; Andrew Steckl

doi:10.1117/12.621954

18 August 2005 Molecular binding and enhanced photoluminescence of bromocresol purple in marine derived DNA

Darnell E. Diggs, Josh Hagen, Zhou Yu, Emily Heckman, F. Kenneth Hopkins, James G. Grote, Andrew Steckl

Proceedings Volume 5934, Nonlinear Optical Transmission and Multiphoton Processes in Organics III; 593407 (2005) https://doi.org/10.1117/12.621954
Event: Optics and Photonics 2005, 2005, San Diego, California, United States

Abstract

Deoxyribonucleic acid (DNA) extracted and purified from salmon roe and milt sacs, a waste product of the fishing industry was studied for molecular binding and photoluminescence effects using bromocresol purple (BCP). Since BCP is both water and alcohol soluble it was investigated for binding efficiency in DNA/water solutions and modified DNA-CTMA/butanol solutions. Circular dichroism studies show that there is a maximum binding concentration of BCP in the DNA/water solution at ~5% by weight of BCP:DNA. In contrast, DNA-CTMA/butanol solutions showed increased binding concentrations up through 10wt% BCP:DNA-CTMA. This apparent binding affinity of DNA-CTMA for BCP also resulted in a significantly higher (6x) photoluminescence in thin film form when compared to BCP:PMMA films of the same doping concentration.

Citation Download Citation

Darnell E. Diggs, Josh Hagen, Zhou Yu, Emily Heckman, F. Kenneth Hopkins, James G. Grote, and Andrew Steckl "Molecular binding and enhanced photoluminescence of bromocresol purple in marine derived DNA", Proc. SPIE 5934, Nonlinear Optical Transmission and Multiphoton Processes in Organics III, 593407 (18 August 2005); https://doi.org/10.1117/12.621954

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