Quantitative doping of commercial polystyrene microbeads with quantum dots

Hai-Qiao Wang; Zhen-Li Huang; Tian-Cai Liu; Jian-Hao Wang; Yuan-Di Zhao; Qing-Ming Luo

doi:10.1117/12.667679

2 February 2006 Quantitative doping of commercial polystyrene microbeads with quantum dots

Hai-Qiao Wang, Zhen-Li Huang, Tian-Cai Liu, Jian-Hao Wang, Yuan-Di Zhao, Qing-Ming Luo

Proceedings Volume 6026, ICO20: Biomedical Optics; 60261J (2006) https://doi.org/10.1117/12.667679
Event: ICO20:Optical Devices and Instruments, 2005, Changchun, China

Abstract

Microbeads with embedded encode characteristics are of considerable interest due to their potential use in multiplexed bioassays, high-throughput screening and combinatorial chemistry. Lots of encoding strategies for tagging or labeling microbeads have arisen in the past couple of years. Compared with the organic dye counterparts, the ideal optical properties of quantum dots (QDs) (e.g. characteristic narrow and symmetric spectra, size-tunable emission and simultaneous excitation) make it possible to tag microbeads in a quantitative way. In this paper, quantitative doping of commercial polystyrene microbeads with single color quantum dots was reported. A detailed analysis of the optical characteristics of the QD-tagged microbeads was presented based on the combination of fluorescent spectroscopy and microscope imaging.

Citation Download Citation

Hai-Qiao Wang, Zhen-Li Huang, Tian-Cai Liu, Jian-Hao Wang, Yuan-Di Zhao, and Qing-Ming Luo "Quantitative doping of commercial polystyrene microbeads with quantum dots", Proc. SPIE 6026, ICO20: Biomedical Optics, 60261J (2 February 2006); https://doi.org/10.1117/12.667679

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