How could the light fluence rate influence the tumor cell-killing rate in photodynamic therapy?

Mingzhao Lee; Tao Xu

doi:10.1117/12.709844

27 October 2006 How could the light fluence rate influence the tumor cell-killing rate in photodynamic therapy?

Mingzhao Lee, Tao Xu

Proceedings Volume 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine; 60470D (2006) https://doi.org/10.1117/12.709844
Event: Fourth International Conference on Photonics and Imaging in Biology and Medicine, 2005, Tianjin, China

Abstract

Some work had been done in this paper to study the role of the fluence rate of the illuminating light in the photodynamic therapy (PDT), which focused on the influence of light fluence rate on the microvasculature damage, the cell killing and the photodynamic reaction impetus. The microvasculature damage was studied through observing the values of RBC's column diameter during the process of the HpD mediated PDT. It was found that less microvasculature damage was induced by 75 mW/cm² illumination than that by 150 mW/cm², indicating that under 75mW/cm² illumination tumor oxygen can be better preserved than 150 mW/cm². The cell killing experiment was performed in vitro and designed in the manner that cell killing rate was only influenced by photobleaching. Higher cell killing rate caused by 75 mW/cm illumination indicated that lower fluence rate light could enhance the light absorbency and decrease the bleaching of photosensitizer. So the cell-killing rate under low fluence rate was enhanced through the oxygen preservation and photobleaching decreasing.

Citation Download Citation

Mingzhao Lee and Tao Xu "How could the light fluence rate influence the tumor cell-killing rate in photodynamic therapy?", Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60470D (27 October 2006); https://doi.org/10.1117/12.709844

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