An electrochemiluminescence non-PCR method for the detection of genetically modified organisms

Jinfeng Liu; Da Xing; Debin Zhu

doi:10.1117/12.710937

27 October 2006 An electrochemiluminescence non-PCR method for the detection of genetically modified organisms

Jinfeng Liu, Da Xing, Debin Zhu

Proceedings Volume 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine; 60473D (2006) https://doi.org/10.1117/12.710937
Event: Fourth International Conference on Photonics and Imaging in Biology and Medicine, 2005, Tianjin, China

Abstract

An electrochemiluminescence non-PCR method has been developed for the detection of genetically modified organisms (GMOs) in crops. Genomic DNA of GMOs was digested with two restriction endonucleases (FOK I and BsrD I), and hybridized with three Ru(bpy)₃²⁺ (TBR)-labeled and one biotinylated probes. The hybridization products were captured onto streptavidin-coated paramagnetic beads, and detected by measuring the electrochemiluminescence (ECL) signal of the TBR label. Whether the tobaccos contain GM components was discriminated by detecting the ECL signal of CaMV35S promoter. The experiment results show that the detection limit for CaMV35S promoter is 100 fmol, and the GM components can be clearly identified in GM tobaccos. The ECL non-PCR method will provide a new means in GMOs detection due to its safety, simplicity and high efficiency.

Citation Download Citation

Jinfeng Liu, Da Xing, and Debin Zhu "An electrochemiluminescence non-PCR method for the detection of genetically modified organisms", Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60473D (27 October 2006); https://doi.org/10.1117/12.710937

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