Time-lapse microscopy studies of bystander effects induced by photosensitization

Yin-Chu Chen; Robert W. Redmond

doi:10.1117/12.673641

23 February 2006 Time-lapse microscopy studies of bystander effects induced by photosensitization

Yin-Chu Chen, Robert W. Redmond

Proceedings Volume 6087, Biophotonics and Immune Responses; 60870S (2006) https://doi.org/10.1117/12.673641
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Reactive oxygen species (ROS) are involved in the pathogenesis of many critical diseases and are also utilized as cytotoxic agents in a variety of treatments for eradication of diseased tissue, including cancer. Oxidative stress ensues when the level of ROS in a system exceeds the antioxidant capacity. Oxidative stress can have local (direct) and long-range (bystander) effects in cells and tissue and this research was carried out to determine the spatial and temporal nature of the photosensitized bystander effect using time-lapse fluorescence microscopy. By initiating photosensitization in only a portion of the microscopic imaging field it was possible to differentiate direct from bystander effects in EMT-6 murine breast cancer cells in 6-well plates. Elevated ROS levels are seen immediately following photodynamic treatment in direct cells with a delayed increase in oxidative stress observed in bystander cells. Cytotoxicity is also seen at earlier times in direct cells and occurs in bystander cells in a delayed fashion. These studies confirm the existence of a bystander effect following photosensitization and implicate mediators capable of diffusing in an intercellular manner from directly photosensitized cells to bystander cells and also implicate increased oxidative stress as a mechanistic factor in generating damage in bystander cells.

Citation Download Citation

Yin-Chu Chen and Robert W. Redmond "Time-lapse microscopy studies of bystander effects induced by photosensitization", Proc. SPIE 6087, Biophotonics and Immune Responses, 60870S (23 February 2006); https://doi.org/10.1117/12.673641

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