Design, synthesis, and evaluation of near-infrared fluorescent pH indicators at physiological range

Zongren Zhang; Duanwen Shen; Kexian Liang; Samuel Achilefu

doi:10.1117/12.647852

15 February 2006 Design, synthesis, and evaluation of near-infrared fluorescent pH indicators at physiological range

Zongren Zhang, Duanwen Shen, Kexian Liang, Samuel Achilefu

Proceedings Volume 6097, Optical Molecular Probes for Biomedical Applications; 60970A (2006) https://doi.org/10.1117/12.647852
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Biochemical processes frequently involve protonation and deprotonation, resulting in pH changes that can be monitored with pH indicators. In heterogenous media such as tissue where indicator concentration or visual observation is limited, highly sensitive pH indicators with reduced tissue autofluorescence are needed. Because of the reduced tissue autofluorescence in the near infrared (NIR) region, NIR fluorescence dyes such as indocyanine green (ICG) and its derivatives have been used to image molecular processes. Removing one of the N-subsitituent of ICG led to pHsensitive dyes operating in the NIR region and at physiologically relevant range. Further modification of the compound and synthetic procedure allowed their conjugation to peptides for specific delivery to target cells and tissues. Synthetic strategy and preliminary results on the spectral and biological properties of such dyes and their bioconjugates are described.

Citation Download Citation

Zongren Zhang, Duanwen Shen, Kexian Liang, and Samuel Achilefu "Design, synthesis, and evaluation of near-infrared fluorescent pH indicators at physiological range", Proc. SPIE 6097, Optical Molecular Probes for Biomedical Applications, 60970A (15 February 2006); https://doi.org/10.1117/12.647852

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