Raman-Spectroscopy for a rapid identification of single microorganisms

Jürgen Popp; Petra Rösch; Michaela Harz; Michael Schmitt; Klaus-Dieter Peschke; Olaf Ronneberger; Hans Burkhardt

doi:10.1117/12.675853

18 April 2006 Raman-Spectroscopy for a rapid identification of single microorganisms

Jürgen Popp, Petra Rösch, Michaela Harz, Michael Schmitt, Klaus-Dieter Peschke, Olaf Ronneberger, Hans Burkhardt

Proceedings Volume 6180, Photonics, Devices, and Systems III; 618024 (2006) https://doi.org/10.1117/12.675853
Event: Photonics, Devices, and Systems III, 2005, Prague, Czech Republic

Abstract

A rapid analysis of microorganisms is necessary for medical, pharmaceutical or food technology applications to identify harmful bacteria. Conventional identification methods require pure cultures from isolates and are often time demanding. Raman spectroscopy offers an alternative approach to identify microorganisms. With Raman microscopy it is possible to measure structures in the sub micrometer range, and therefore single bacteria cells are accessible. Micro-Raman mapping experiments proof that the bacterium shows a spatial homogeneity, since bacteria normally exhibit no compartments, therefore one spectrum of a single vegetative bacterial cell is sufficient to identify the strain. In contrary bacterial spores and yeast cells exhibit a high spatial dependency of the observed Raman spectra. For heterogeneous samples like single spores or yeast cells a mean spectrum from up to ten different positions is required to describe the complete cell. Using micro-Raman spectra of single bacterial cells and average spectra of yeast cells it is possible to create a database and identify microorganisms on species or even strain level.

Citation Download Citation

Jürgen Popp, Petra Rösch, Michaela Harz, Michael Schmitt, Klaus-Dieter Peschke, Olaf Ronneberger, and Hans Burkhardt "Raman-Spectroscopy for a rapid identification of single microorganisms", Proc. SPIE 6180, Photonics, Devices, and Systems III, 618024 (18 April 2006); https://doi.org/10.1117/12.675853

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