Optical tracking of micro-objects within living cells

J. Ježek; M. Vařecha; J. Amrichova; M. Kozubek; T. Čižmár; P. Zemánek

doi:10.1117/12.675855

18 April 2006 Optical tracking of micro-objects within living cells

J. Ježek, M. Vařecha, J. Amrichova, M. Kozubek, T. Čižmár, P. Zemánek

Proceedings Volume 6180, Photonics, Devices, and Systems III; 618025 (2006) https://doi.org/10.1117/12.675855
Event: Photonics, Devices, and Systems III, 2005, Prague, Czech Republic

Abstract

Tracing of foreign objects inside living cells is very exciting way how to study interior of living objects in nondestructive way. We imported fluorescent submicron particles into the living cells using liposomes as carriers to study the local mechanical heterogeneity of the cell cytoplasm. Thermal motion of these probes within the cell is tracked using fluorescent video-microscopy. The time-records of the probe positions reveal their trajectories and accessible space to the probes inside the cytoplasm of living cells. Further analyses of the thermal motion of the probes can reveal the mechanism of sub-cellular transport and properties of the cytoplasm in vivo.

Citation Download Citation

J. Ježek, M. Vařecha, J. Amrichova, M. Kozubek, T. Čižmár, and P. Zemánek "Optical tracking of micro-objects within living cells", Proc. SPIE 6180, Photonics, Devices, and Systems III, 618025 (18 April 2006); https://doi.org/10.1117/12.675855

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