Phantom and in-vivo measurements of hemoglobin concentration and oxygen saturation using PCT-S small animal scanner

Bo Liu; Daniel Reinecke; Robert A. Kruger; Keith M. Stantz

doi:10.1117/12.701376

13 February 2007 Phantom and in-vivo measurements of hemoglobin concentration and oxygen saturation using PCT-S small animal scanner

Bo Liu, Daniel Reinecke, Robert A. Kruger, Keith M. Stantz

Author Affiliations +

Proceedings Volume 6437, Photons Plus Ultrasound: Imaging and Sensing 2007: The Eighth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics; 64371X (2007) https://doi.org/10.1117/12.701376
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Purpose: The purpose of this study is to calibrate the PCT small animal scanner system with a blood phantom and to measure the blood hemoglobin concentration and oxygen saturation level in mouse tail vein and tumors. Methods and Materials: A blood phantom with variable blood flow and oxygen content was integrated into the PCT scanner with a circulation system. The circulation system consisted of a pump, an oxygen electrode detector and a tonometer. The SaO2 values were determined based on a linear combination model of oxy-hemoglobin and deoxy-hemoglobin absorption spectrum curves. Hemoglobin concentration (CHb) was determined by measuring the linear relationship for different blood dilutions. SaO2 and CHb as measured in vivo for the artery and vein in a mouse tail were also measured. Results: The PCT spectra of blood phantom samples were measured and compared with hemoglobin optical absorption spectra. The linear relationship between hemoglobin concentration and PCT intensities were observed by phantom study. The hemoglobin concentration of mouse is ~9.7g/dL. The saturation difference between arteries and veins in mouse tail is also measured by PCT scan. Conclusions: Both the phantom and living mouse tail vessel scans have shown that NIR PCT-spectroscopy can be used to measure the hemoglobin saturation level and hemoglobin concentration in small animal for future tumor hypoxia study.

Citation Download Citation

Bo Liu, Daniel Reinecke, Robert A. Kruger, and Keith M. Stantz "Phantom and in-vivo measurements of hemoglobin concentration and oxygen saturation using PCT-S small animal scanner", Proc. SPIE 6437, Photons Plus Ultrasound: Imaging and Sensing 2007: The Eighth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, 64371X (13 February 2007); https://doi.org/10.1117/12.701376

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