Second-harmonic generation polarization microscopy by rotation of excitation light

Peter Tramyeon Fwu; Chen-Kuan Chou; Wei-Liang Chen; Chen-Yuan Dong

doi:10.1117/12.699458

12 February 2007 Second-harmonic generation polarization microscopy by rotation of excitation light

Peter Tramyeon Fwu, Chen-Kuan Chou, Wei-Liang Chen, Chen-Yuan Dong

Proceedings Volume 6442, Multiphoton Microscopy in the Biomedical Sciences VII; 64421C (2007) https://doi.org/10.1117/12.699458
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

When imaging anisotropic samples with a laser scanning optical microscope, the results are often affected by the polarization of the excitation light source. Quantifying the polarization dependence of biological fibrous material such as muscle and collagen allows us to gain molecular information at length scale below the resolution of optical microscopes. One problem associated with rotating the direction of linearly polarized excitation light for an epi-illuminated laser scanning microscope is due to the reflective properties of the main dichroic mirror. Depending on the direction of the incident polarization, the dichroic mirror can induce different amount of phase retardation, thus altering the desired output polarization. In this work, we theoretically determined the needed combination of wave plates and their angular positions to compensate for the effect of the dichroic mirror, thus achieving any arbitrary linear polarization angle for the excitation incident on sample.

Citation Download Citation

Peter Tramyeon Fwu, Chen-Kuan Chou, Wei-Liang Chen, and Chen-Yuan Dong "Second-harmonic generation polarization microscopy by rotation of excitation light", Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 64421C (12 February 2007); https://doi.org/10.1117/12.699458

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