Imaging ligand-gated ion channels with quantum dots

I. D. Tomlinson; Rebecca L. Orndorff; Hélène Gussin; John N. Mason; Randy D. Blakely; David R. Pepperberg; Sandra J. Rosenthal

doi:10.1117/12.713696

27 February 2007 Imaging ligand-gated ion channels with quantum dots

I. D. Tomlinson, Rebecca L. Orndorff, Hélène Gussin, John N. Mason, Randy D. Blakely, David R. Pepperberg, Sandra J. Rosenthal

Author Affiliations +

Proceedings Volume 6448, Colloidal Quantum Dots for Biomedical Applications II; 64480X (2007) https://doi.org/10.1117/12.713696
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

In this paper we report two different methodologies for labeling ligand-gated receptors. The first of these builds upon our earlier work with serotonin conjugated quantum dots and our studies with pegilated quantum dots to reduce non specific binding. In this approach a pegilated derivative of muscimol was synthesized and attached via an amide linkage to quantum dots coated in an amphiphillic polymer derivative of poly acrylamide. These conjugates were used to image the GABA_C receptor in oocytes. An alternative approach was used to image tissue sections to study nicotinic acetylcholine receptors in the neuro muscular junction with biotinylated Bungerotoxin and streptavidin coated quantum dots.

Citation Download Citation

I. D. Tomlinson, Rebecca L. Orndorff, Hélène Gussin, John N. Mason, Randy D. Blakely, David R. Pepperberg, and Sandra J. Rosenthal "Imaging ligand-gated ion channels with quantum dots", Proc. SPIE 6448, Colloidal Quantum Dots for Biomedical Applications II, 64480X (27 February 2007); https://doi.org/10.1117/12.713696

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