An adaptive optics biomicroscope for mouse retinal imaging

David P. Biss; Robert H. Webb; Yaopeng Zhou; Thomas G. Bifano; Parisa Zamiri M.D.; Charles P. Lin

doi:10.1117/12.707531

6 February 2007 An adaptive optics biomicroscope for mouse retinal imaging

David P. Biss, Robert H. Webb, Yaopeng Zhou, Thomas G. Bifano, Parisa Zamiri M.D., Charles P. Lin

Proceedings Volume 6467, MEMS Adaptive Optics; 646703 (2007) https://doi.org/10.1117/12.707531
Event: MOEMS-MEMS 2007 Micro and Nanofabrication, 2007, San Jose, California, United States

Abstract

In studying retinal disease on a microscopic level, in vivo imaging has allowed researchers to track disease progression in a single animal over time without sacrificing large numbers of animals for statistical studies. Historically, a drawback of in vivo retinal imaging, when compared to ex vivo imaging, is decreased image resolution due to aberrations present in the mouse eye. Adaptive optics has successfully corrected phase aberrations introduced the eye in ophthalmic imaging in humans. We are using adaptive optics to correct for aberrations introduced by the mouse eye in hopes of achieving cellular resolution retinal images of mice in vivo. In addition to using a wavefront sensor to drive the adaptive optic element, we explore the using image data to correct for wavefront aberrations introduced by the mouse eye. Image data, in the form of the confocal detection pinhole intensity are used as the feedback mechanism to control the MEMS deformable mirror in the adaptive optics system. Correction for wavefront sensing and sensor-less adaptive optics systems are presented.

Citation Download Citation

David P. Biss, Robert H. Webb, Yaopeng Zhou, Thomas G. Bifano, Parisa Zamiri M.D., and Charles P. Lin "An adaptive optics biomicroscope for mouse retinal imaging", Proc. SPIE 6467, MEMS Adaptive Optics, 646703 (6 February 2007); https://doi.org/10.1117/12.707531

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available