Using delayed chemiluminescence as a photodynamic therapy dose metric in vivo

Yanchun Wei; Da Xing; Qun Chen

doi:10.1117/12.741308

1 May 2007 Using delayed chemiluminescence as a photodynamic therapy dose metric in vivo

Yanchun Wei, Da Xing, Qun Chen

Proceedings Volume 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine; 65342H (2007) https://doi.org/10.1117/12.741308
Event: Fifth International Conference on Photonics and Imaging in Biology and Medicine, 2006, Wuhan, China

Abstract

Photodynamic therapy (PDT) is an important method to treat tumor. It is known that singlet oxygen (¹O₂) is the main factor mediating cytotoxicity in PDT. The effectiveness of a PDT treatment is directly linked to the ¹O₂ produced in the target. So to control the dose of ¹O₂ is very important. Although the luminescence from ¹O₂ can be detected and is suggested as an indicator for evaluating photodynamic therapy, the inherited disadvantages limit its potential for in vivo applications. We have previously reported that chemiluminescence (CL) can be used to detect ¹O₂ production in PDT and linked the signal to the cytotoxicity, but the irradiation of laser decrease the sensitivity of the detection in vivo. During PDT the high sensitivity probe, Fluoresceinyl Cypridina Luciferin Analog (FCLA), is used to monitor ¹O₂. In order to avoid the infection of irradiation light, the delayed CL of FCLA is used to indicate ¹O₂. After recording the delayed CL during PDT and scoring the skin of mice after PDT, the statistic analysis was done. The data shows a remarkable relationship between the score and the CL. the result suggests that the CL can be used as a dose metric in vivo in PDT.

Citation Download Citation

Yanchun Wei, Da Xing, and Qun Chen "Using delayed chemiluminescence as a photodynamic therapy dose metric in vivo", Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65342H (1 May 2007); https://doi.org/10.1117/12.741308

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Photodynamic therapy

In vivo imaging

Chemiluminescence

Oxygen

Luminescence

Skin

Tumors

Show All Keywords

Keywords/Phrases

Search In:

Publication Years