Fluorescence detection of esophageal neoplasia

E. Borisova; B. Vladimirov; L. Avramov

doi:10.1117/12.803962

9 June 2008 Fluorescence detection of esophageal neoplasia

E. Borisova, B. Vladimirov, L. Avramov

Proceedings Volume 6791, Saratov Fall Meeting 2007: Optical Technologies in Biophysics and Medicine IX; 679104 (2008) https://doi.org/10.1117/12.803962
Event: Saratov Fall Meeting 2007: Optical Technologies in Biophysics and Medicine IX, 2006, Saratov, Russian Federation

Abstract

White-light endoscopy is well-established and wide used modality. However, despite the many technological advances that have been occurred, conventional endoscopy is suboptimal and usually detects advanced stage lesions. The limitations of standard endoscopy initiate development of spectroscopic techniques, additional to standard endoscopic equipment. One of the most sensitive approaches is fluorescence spectroscopy of gastrointestinal mucosa for neoplasia detection. In the recent study delta-aminolevulinic acid/Protoporphyrin IX (5-ALA/PpIX) is used as fluorescent marker for dysplasia and tumor detection in esophagus. The 5-ALA is administered per os six hours before measurements at dose 20 mg/kg weight. Excitation source has max of emission at 405 nm and light is delivered by the standard light guide of the endoscopic equipment. Through endoscopic instrumental channel a fiber is applied to return information about fluorescence to microspectrometer. Spectral features observed during endoscopic investigations could be distinct as the next regions: 450-630 nm region, where tissue autofluorescence is observed; 630-710 nm region, where fluorescence of PpIX is clearly pronounced; 530-580 nm region, where minima in the autofluorescence signal are observed, related to reabsorption of blood. The lack of fluorescence peaks in the red spectral area for normal mucosa is an indication for selective accumulation of 5-ALA/PpIX only in abnormal sites Very good correlation between fluorescence signals and histology examination of the lesions investigated is achieved.

Citation Download Citation

E. Borisova, B. Vladimirov, and L. Avramov "Fluorescence detection of esophageal neoplasia", Proc. SPIE 6791, Saratov Fall Meeting 2007: Optical Technologies in Biophysics and Medicine IX, 679104 (9 June 2008); https://doi.org/10.1117/12.803962

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