Automatic compensation for phase aberration in digital holographic microscopy

Guangjun Wang; Huaying Wang; Jie Zhao; Qinglei Su; Dayong Wang

doi:10.1117/12.756768

4 January 2008 Automatic compensation for phase aberration in digital holographic microscopy

Guangjun Wang, Huaying Wang, Jie Zhao, Qinglei Su, Dayong Wang

Proceedings Volume 6832, Holography and Diffractive Optics III; 683214 (2008) https://doi.org/10.1117/12.756768
Event: Photonics Asia 2007, 2007, Beijing, China

Abstract

Based on the point spread function of the off-axis Fresnel digital holographic system with pre-magnification, the phase aberration introduced by microscope objective is obtained. Using a collimated light as reconstructing wave, the phase aberration introduced by the difference between the reconstructing wave and the reference recording wave is analyzed. This method is very simple, and it is very different from the one proposed by T. Colomb et al. The phase mask that can be used to compensate automatically the phase aberration in the phase reconstruction is obtained. According to the principle of digital holography, it must be pointed out that for Fresnel digital holography, the recording distance must be determined accurately before compensating automatically the phase aberration. This is done by an automatic focusing procedure, which is based on image-gray-entropy-method. The simulation result for a special three-dimensional micro object, which is polluted by a random noise, is presented. The percentage error of the reconstructing distance obtained by the focusing procedure is below 0.6 for SNR = 25. Then an automatic aberration compensation procedure, which is the same as that one proposed by T. Colomb et al., is applied to reconstruct the phase image. The results show that for a weak noise the above method is very effective; for a stronger noise the procedure described here is applied iteratively, starting from the initial values provided by the first evaluation; while for a very strong noise the procedure fails at all. Moreover, after applying a median filter to the primary reconstructed phase image, the aberration of the phase image obtained by further iteration decreases, at the same time the noise is strengthen.

Citation Download Citation

Guangjun Wang, Huaying Wang, Jie Zhao, Qinglei Su, and Dayong Wang "Automatic compensation for phase aberration in digital holographic microscopy", Proc. SPIE 6832, Holography and Diffractive Optics III, 683214 (4 January 2008); https://doi.org/10.1117/12.756768

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Digital holography

Signal to noise ratio

3D image reconstruction

Holography

Holograms

Digital filtering

Charge-coupled devices

Show All Keywords

Keywords/Phrases

Search In:

Publication Years