Micro amperometric immunosensor by antibody immobilizing with electropolymerized protein A

Chao Bian; Jizhou Sun; Lan Qu; Shanhong Xia

doi:10.1117/12.775058

4 January 2008 Micro amperometric immunosensor by antibody immobilizing with electropolymerized protein A

Chao Bian, Jizhou Sun, Lan Qu, Shanhong Xia

Proceedings Volume 6836, MEMS/MOEMS Technologies and Applications III; 68360P (2008) https://doi.org/10.1117/12.775058
Event: Photonics Asia 2007, 2007, Beijing, China

Abstract

A micro plane amperometric immunosensor for the detection of human immunoglobulin G is fabricated on silicon wafer based on Micro-Electro-Mechanical Systems (MEMS) technology. This microsensor is an electrochemical system composed of two-electrode (working electrode and counter electrode) integrated with a micro reaction cell made of SU-8 photoresist. The sensitive area of the working electrode is only 1 mm². A new method for the orientation-controlled immobilization of antibody based on staphylococcal protein A (SPA) and polypyrrole (PPy) is developed, which is fast, controllable and proper to microelectrode. PPy as a transition layer is first electropolymerized at the sensing area of the working electrode. Then SPA is co-electropolymerized with PPy for further orientedy immobilization of antibody. The electrodes modified by PPy and the coelectropolymer of PPy and SPA were characterized by SEM. HIgG range from 5 ng/ml to 640 ng/ml can be detected by this immunosensor with less consumption of reagent and acceptable reproducibility and stability.

Citation Download Citation

Chao Bian, Jizhou Sun, Lan Qu, and Shanhong Xia "Micro amperometric immunosensor by antibody immobilizing with electropolymerized protein A", Proc. SPIE 6836, MEMS/MOEMS Technologies and Applications III, 68360P (4 January 2008); https://doi.org/10.1117/12.775058

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