Ex-vivo multiphoton analysis of rabbit corneal wound healing following photorefractive keratectomy

Tsung-Jen Wang; Wen Lo; Chen-Yuan Dong; Fung-Rong Hu

doi:10.1117/12.762212

11 March 2008 Ex-vivo multiphoton analysis of rabbit corneal wound healing following photorefractive keratectomy

Tsung-Jen Wang, Wen Lo, Chen-Yuan Dong, Fung-Rong Hu

Proceedings Volume 6844, Ophthalmic Technologies XVIII; 684414 (2008) https://doi.org/10.1117/12.762212
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

The aim of this study is to assess the application of multiphoton autofluorescence and second harmonic generation (SHG) microscopy for investigating corneal wound healing after high myopic (-10.0D) photorefractive keratectomy (PRK) procedures on the rabbit eyes. The effect of PRK on the morphology and distribution of keratocytes were investigated using multiphoton excited autofluorescence imaging, while the effect of PRK on the arrangement of collagen fibers was monitored by second-harmonic generation imaging. Without histological processing, multiphoton microscopy is able to characterize corneal damage and wound healing from PRK. Our results show that this technique has potential application in the clinical evaluation of corneal damage due to refractive surgery, and may be used to study the unwanted side effects of these procedures.

Citation Download Citation

Tsung-Jen Wang, Wen Lo, Chen-Yuan Dong, and Fung-Rong Hu "Ex-vivo multiphoton analysis of rabbit corneal wound healing following photorefractive keratectomy", Proc. SPIE 6844, Ophthalmic Technologies XVIII, 684414 (11 March 2008); https://doi.org/10.1117/12.762212

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available