Noninvasive mapping of the electrically stimulated mouse brain using photoacoustic microscopy

Erich W. Stein; Konstantin Maslov; Lihong V. Wang

doi:10.1117/12.769508

28 February 2008 Noninvasive mapping of the electrically stimulated mouse brain using photoacoustic microscopy

Erich W. Stein, Konstantin Maslov, Lihong V. Wang

Proceedings Volume 6856, Photons Plus Ultrasound: Imaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics; 68561J (2008) https://doi.org/10.1117/12.769508
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Photoacoustic imaging techniques possess high optical contrast with ultrasonic resolution while exceeding imaging depths of pure optical techniques, affording high resolution images deep within scattering biological tissues. In this work, we employ reflection-mode photoacoustic microscopy to non-invasively monitor hemodynamic contrasts and map brain activity. Changes in vascular dynamics of the mouse somatosensory cortex were evoked through electrical stimulation of the hindpaw, resulting in increased photoacoustic intensities spatially correlated with contra-lateral vasculature. Results demonstrate the ability to map brain activation with vascular resolution in three-dimensions, as well as monitor single-vessel hemodynamics with millisecond temporal resolution. Furthermore, these results implicate the feasibility of photoacoustic microscopy to probe intra-cortical single-vessel hemodynamics and pave the way for more extensive functional brain imaging studies.

Citation Download Citation

Erich W. Stein, Konstantin Maslov, and Lihong V. Wang "Noninvasive mapping of the electrically stimulated mouse brain using photoacoustic microscopy", Proc. SPIE 6856, Photons Plus Ultrasound: Imaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, 68561J (28 February 2008); https://doi.org/10.1117/12.769508

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