Development of fluorescent contrast agents for optical imaging of mouse disease models

J. Kovar; M. Simpson; A. Schutz-Geschwender; X. Xu; W. M. Volcheck; E. Sevick-Muraca; D. M. Olive

doi:10.1117/12.760815

22 February 2008 Development of fluorescent contrast agents for optical imaging of mouse disease models

J. Kovar, M. Simpson, A. Schutz-Geschwender, X. Xu, W. M. Volcheck, E. Sevick-Muraca, D. M. Olive

Proceedings Volume 6867, Molecular Probes for Biomedical Applications II; 686707 (2008) https://doi.org/10.1117/12.760815
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Optical imaging is a rapidly developing field of research aimed at non-invasively interrogating animals for disease progression, determining the effects of a drug on a particular pathology, assessing the pharmacokinetic behavior of a drug, or identifying molecular biomarkers of disease. One of the key components of molecular imaging is the development of specific, targeted imaging contrast agents to assess these biological processes. The development of robust fluorochrome-labeled optical agents is a process that is often underestimated in terms of its complexity. We describe here the development process and performance issues for three different optical agents: IRDye 800CW EGF (epidermal growth factor), IRDye^(R) 800CW 2-DG (2-deoxy D-glucose), and an IRDye 680 BoneTag^TM. In vitro competitive assays were developed for two of the markers to demonstrate specificity. Specificity was confirmed in animal studies. Uptake of IRDye 800CW 2-DG was also examined by near-infrared confocal microscopy. Histological examinations were performed on target and non-target tissues following the completion of the imaging studies. The issues unique to the development of each labeled marker are discussed.

Citation Download Citation

J. Kovar, M. Simpson, A. Schutz-Geschwender, X. Xu, W. M. Volcheck, E. Sevick-Muraca, and D. M. Olive "Development of fluorescent contrast agents for optical imaging of mouse disease models", Proc. SPIE 6867, Molecular Probes for Biomedical Applications II, 686707 (22 February 2008); https://doi.org/10.1117/12.760815

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