Microscopic heterogeneity vs. macroscopic homogeneity in tissue phantoms using reflectance-mode confocal scanning laser microscopy

Ravikant Samatham; David Levitz; Reid Fletcher; Yongji Fu; Steven L. Jacques

doi:10.1117/12.764073

21 February 2008 Microscopic heterogeneity vs. macroscopic homogeneity in tissue phantoms using reflectance-mode confocal scanning laser microscopy

Ravikant Samatham, David Levitz, Reid Fletcher, Yongji Fu, Steven L. Jacques

Author Affiliations +

Proceedings Volume 6870, Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue; 68700H (2008) https://doi.org/10.1117/12.764073
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Preparation of phantoms with reproducible and homogenous optical properties is tricky. The microscopic heterogeneity and macroscopic homogeneity of tissue phantoms were compared using reflectance-mode confocal laser scanning microscopy. Tissue phantoms were prepared using polystyrene microspheres as scattering medium in aqueous and gel matrix. Uniform distribution of microparticles in phantoms was evaluated by confocal imaging. Comparison of the heterogeneity of the phantoms was accomplished based on microscopic optical scattering properties. Distribution of optical properties at the microscopic levels was determined by a simple theory developed based on the depth-dependent decay of the reflectance-mode confocal signal. The variability of these optical properties is correlated to heterogeneity of the phantom. These microscopic properties were compared with macroscopic properties determined by ballistic transmission experiment. This enabled to optimize the phantom preparation procedure.

Citation Download Citation

Ravikant Samatham, David Levitz, Reid Fletcher, Yongji Fu, and Steven L. Jacques "Microscopic heterogeneity vs. macroscopic homogeneity in tissue phantoms using reflectance-mode confocal scanning laser microscopy", Proc. SPIE 6870, Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue, 68700H (21 February 2008); https://doi.org/10.1117/12.764073

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KEYWORDS

Scattering

Confocal microscopy

Optical properties

Laser scattering

Optical spheres

Particles

Anisotropy

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