Cytometric biochips with optically active surfaces for spatial engineering of fluorescence excitation

Huw D. Summers; Rachel J. Errington; Paul Smith; Daniel R. Matthews; James F. Leary

doi:10.1117/12.763645

19 February 2008 Cytometric biochips with optically active surfaces for spatial engineering of fluorescence excitation

Huw D. Summers, Rachel J. Errington, Paul Smith, Daniel R. Matthews, James F. Leary

Proceedings Volume 6886, Microfluidics, BioMEMS, and Medical Microsystems VI; 688603 (2008) https://doi.org/10.1117/12.763645
Event: MOEMS-MEMS 2008 Micro and Nanofabrication, 2008, San Jose, California, United States

Abstract

The development of surface-active biochips for control of fluorescence within microscopy platforms is described. These use surface-plasmon control to provide selective excitation of fluorescently labeled, live cell populations. These chips effectively combine a number of commonly used techniques such as SPR, TIRF and epi-fluorescence within a single device and have the potential to provide sub-cellular discrimination of excitation in 3-D. Thus within a single field of view we can selectively excite membrane versus cytoplasm and localise the excitation within the lateral plane to an area of a few square microns.

Citation Download Citation

Huw D. Summers, Rachel J. Errington, Paul Smith, Daniel R. Matthews, and James F. Leary "Cytometric biochips with optically active surfaces for spatial engineering of fluorescence excitation", Proc. SPIE 6886, Microfluidics, BioMEMS, and Medical Microsystems VI, 688603 (19 February 2008); https://doi.org/10.1117/12.763645

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