Direct electrical detection of target cells on a microfluidic biochip

Mehdi Javanmard; AmirAli H. Talasaz; Mohsen Nemat-Gorgani; Fabian Pease; Mostafa Ronaghi; Ronald W. Davis

doi:10.1117/12.765611

12 February 2008 Direct electrical detection of target cells on a microfluidic biochip

Mehdi Javanmard, AmirAli H. Talasaz, Mohsen Nemat-Gorgani, Fabian Pease, Mostafa Ronaghi, Ronald W. Davis

Proceedings Volume 6886, Microfluidics, BioMEMS, and Medical Microsystems VI; 68860B (2008) https://doi.org/10.1117/12.765611
Event: MOEMS-MEMS 2008 Micro and Nanofabrication, 2008, San Jose, California, United States

Abstract

Pathogenic bacterial cell detection is currently performed using techniques such as culture enrichment and various plating methods, which are expensive and can take up to several days. In this study, we describe the design, fabrication, and testing of a rapid and inexpensive sensor for detection of target cells electrically in real-time. The sensor operates with the use of microelectrodes integrated in a micro-channel. As a proof of principle, we have successfully demonstrated real-time detection of target yeast cells with a concentration of 10⁷ cells/ml. We have also demonstrated the selectivity of our sensors in responding to target cells while remaining irresponsive to non-target cells. We also perform theoretical modeling in order to determine the ultimate detection limit of the sensor. Based on our modeling results, proper optimization of the sensor can yield detection limits approaching the single cell level.

Citation Download Citation

Mehdi Javanmard, AmirAli H. Talasaz, Mohsen Nemat-Gorgani, Fabian Pease, Mostafa Ronaghi, and Ronald W. Davis "Direct electrical detection of target cells on a microfluidic biochip", Proc. SPIE 6886, Microfluidics, BioMEMS, and Medical Microsystems VI, 68860B (12 February 2008); https://doi.org/10.1117/12.765611

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available