The role of reactive oxygen species in PDT efficacy

Michael Price; Nakaiya Okan-Mensah; Ann Marie Santiago; David Kessel

doi:10.1117/12.810231

18 February 2009 The role of reactive oxygen species in PDT efficacy

Michael Price, Nakaiya Okan-Mensah, Ann Marie Santiago, David Kessel

Proceedings Volume 7164, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVIII; 716402 (2009) https://doi.org/10.1117/12.810231
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

While the first reactive oxygen species (ROS) formed during photodynamic therapy (PDT) is singlet molecular oxygen (¹O₂), other ROS are formed downstream including superoxide anion radical (•CO₂ ^-), hydrogen peroxide (H₂O₂) and hydroxyl radical (•OH). In this study, we examined the role of H₂O₂ in the phototoxic response to PDT in murine leukemia L388 cells. Inhibition of catalase activity, a major pathway to H₂O₂ detoxification, led to enhanced apoptosis and cell death. Addition of exogenous catalase offered protection from phototoxicity as did chelation of Fe⁺², a co-factor in •OH production from H₂O₂. These results indicate the H₂O₂ formed during PDT plays a role in PDT efficacy.

Citation Download Citation

Michael Price, Nakaiya Okan-Mensah, Ann Marie Santiago, and David Kessel "The role of reactive oxygen species in PDT efficacy", Proc. SPIE 7164, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVIII, 716402 (18 February 2009); https://doi.org/10.1117/12.810231

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