Reconstruction of thin fluorophore-filled capillaries in thick scattering medium using fluorescence diffuse optical tomography within the diffusion approximation

Johanne Desrochers; Patrick Vermette; Réjean Fontaine; Yves Bérubé-Lauzière

doi:10.1117/12.809870

25 February 2009 Reconstruction of thin fluorophore-filled capillaries in thick scattering medium using fluorescence diffuse optical tomography within the diffusion approximation

Johanne Desrochers, Patrick Vermette, Réjean Fontaine, Yves Bérubé-Lauzière

Author Affiliations +

Proceedings Volume 7179, Optics in Tissue Engineering and Regenerative Medicine III; 71790I (2009) https://doi.org/10.1117/12.809870
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Current efforts in tissue engineering target the growth of 3D volumes of tissue cultures in bioreactor conditions. Fluorescence optical tomography has the potential to monitor cells viability and tissue growth non-destructively directly within the bioreactor via bio-molecular fluorescent labelling strategies. We currently work on developing the imaging instrumentation for tissue cultures in bioreactor conditions. Previously, we localized in 3D thin fluorescent-labelled capillaries in a cylindrically shaped bioreactor phantom containing a diffusive medium with our time-of-flight localization technique. Here, we present our first reconstruction results of the spatial distribution of fluorophore concentrations for labelled capillaries embedded in a bioreactor phantom.

Citation Download Citation

Johanne Desrochers, Patrick Vermette, Réjean Fontaine, and Yves Bérubé-Lauzière "Reconstruction of thin fluorophore-filled capillaries in thick scattering medium using fluorescence diffuse optical tomography within the diffusion approximation", Proc. SPIE 7179, Optics in Tissue Engineering and Regenerative Medicine III, 71790I (25 February 2009); https://doi.org/10.1117/12.809870

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