Magnetic DNA separation process with functionalized magnetic silica nanoparticles

Jiho Lee; Jeong Ho Chang

doi:10.1117/12.814120

30 December 2008 Magnetic DNA separation process with functionalized magnetic silica nanoparticles

Jiho Lee, Jeong Ho Chang

Proceedings Volume 7270, Biomedical Applications of Micro- and Nanoengineering IV and Complex Systems; 72701C (2008) https://doi.org/10.1117/12.814120
Event: SPIE Smart Materials, Nano- and Micro-Smart Systems, 2008, Melbourne, Australia

Abstract

Work presented here describes a simple and convenient process to highly efficient and direct DNA separation with functionalized silica-coated magnetic nanoparticles. Iron oxide magnetic nanoparticles and silica-coated magnetic nanoparticles were obtained uniformly and the silica coating thickness could be easily controlled in a range from 10 to 50 nm by changing the concentration of silica precursor (TEOS) including the controlled magnetic strength and particle size. A change in the surface hydrophilicity on the nanoparticles was introduced by aminosilanization to enhance the selective DNA separation resulting from electrostatic interaction. The efficiency of the DNA separation was explored via the function of the amino-group numbers, particle size, the amount of the nanoparticles used, and the concentration of NaCl salt.

Citation Download Citation

Jiho Lee and Jeong Ho Chang "Magnetic DNA separation process with functionalized magnetic silica nanoparticles", Proc. SPIE 7270, Biomedical Applications of Micro- and Nanoengineering IV and Complex Systems, 72701C (30 December 2008); https://doi.org/10.1117/12.814120

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