Delay, change and bifurcation of the immunofluorescence distribution attractors in health statuses diagnostics and in medical treatment

Nikolay E. Galich; Michael V. Filatov

doi:10.1117/12.836452

17 June 2009 Delay, change and bifurcation of the immunofluorescence distribution attractors in health statuses diagnostics and in medical treatment

Nikolay E. Galich, Michael V. Filatov

Author Affiliations +

Proceedings Volume 7377, Twelfth International Workshop on Nanodesign Technology and Computer Simulations; 73770C (2009) https://doi.org/10.1117/12.836452
Event: Nano-Design, Technology, Computer Simulations, 2008, Minsk, Belarus

Abstract

Communication contains the description of the immunology experiments and the experimental data treatment. New nonlinear methods of immunofluorescence statistical analysis of peripheral blood neutrophils have been developed. We used technology of respiratory burst reaction of DNA fluorescence in the neutrophils cells nuclei due to oxidative activity. The histograms of photon count statistics the radiant neutrophils populations' in flow cytometry experiments are considered. Distributions of the fluorescence flashes frequency as functions of the fluorescence intensity are analyzed. Statistic peculiarities of histograms set for healthy and unhealthy donors allow dividing all histograms on the three classes. The classification is based on three different types of smoothing and long-range scale averaged immunofluorescence distributions and their bifurcation. Heterogeneity peculiarities of long-range scale immunofluorescence distributions allow dividing all histograms on three groups. First histograms group belongs to healthy donors. Two other groups belong to donors with autoimmune and inflammatory diseases. Some of the illnesses are not diagnosed by standards biochemical methods. Medical standards and statistical data of the immunofluorescence histograms for identifications of health and illnesses are interconnected. Possibilities and alterations of immunofluorescence statistics in registration, diagnostics and monitoring of different diseases in various medical treatments have been demonstrated. Health or illness criteria are connected with statistics features of immunofluorescence histograms. Neutrophils populations' fluorescence presents the sensitive clear indicator of health status.

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Nikolay E. Galich and Michael V. Filatov "Delay, change and bifurcation of the immunofluorescence distribution attractors in health statuses diagnostics and in medical treatment", Proc. SPIE 7377, Twelfth International Workshop on Nanodesign Technology and Computer Simulations, 73770C (17 June 2009); https://doi.org/10.1117/12.836452

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